Reagents and materials:
1. Primary antibody:
(1) ABCG2----------------- Mouse Monoclonal BXP-21---------1:100
(2) Connexin-43----------- Rabbit polyclonal CX43-------------1:100
(3) cytokeratin 3 (K3)-----mouse monoclonal AE5----------- 1:500
(4) Cytokeratin 12 (K12) --- Rabbit polyclonal J7-------------- 1:200
2. Secondary antibody;
(1) Anti-mouse IgG-------------Alexa-488----------------1:2500
(2) Anti-rabbit IgG---------------Alexa-546----------------1:1500
3. Blocking antibody: 10% heat-inactivated goat serum prepared in PBSA;
4. Trion X-100;
5. PBSA;
6. Paraformaldehyde (PFA), the 16% stock solution is prepared with PBSA to a working concentration of 4%;
7. Immu-Mount anti-fade seal tablets;
8. Slides and #1 coverslips;
9. Confocal microscope;
experimental method:
1. Cells cultured on plastic plates or amniotic membranes;
2. CMF-Saline G is washed once;
3. Fix with freshly prepared 4% PFA for 15 min at room temperature;
4. Wash PBSA once;
5. 0.1% Triton X-100 rupture film for 10min;
6. The sample was incubated with primary antibody for 1 h at room temperature;
7. Wash PBSA twice;
8. Add secondary antibody to incubate for 1 h at room temperature;
9. Wash 2 times with PBSA;
10. #1 coverslips, sealed with a minimum volume of anti-fade seal;
11. Observe the sample with a 40× oil mirror under a fluorescent or confocal microscope;
Excretion is an important part of metabolism, however, due to various reasons, many patients will not be able to auto- urinate normally and smoothly. So we need to help urinate manually. Catheterization is a very common practice of nursing operations after closely observed and documented.
Yingmed offers you a series of catheter-related care products. For example: Silicone catheter, Urine bag, Urinal,Bed Pan .
Through the urine and stool, doctors can judge patients` condition. Yingmed also provided Urinal cups and disposable tubes for storage , then the urine and stool could be delivered to lab to do analysis. In addition, the urine analyzer will help the experimenter to get a correct data .
You will find all of the disposable urinary care products in this category.
Disposable Urethral Catheter,Red Latex Catheter,Red Latex Urethral Catheter,Curving End Foley Catheter,Foley Urine Catheter,Disposbale Ostomy Bag,Urine Collection Bag,Urine Cup
NINGBO YINGMED MEDICAL INSTRUMENTS CO.,LTD , https://www.chinayingmed.com