In modern biology, medicine and translational medicine, pharmacology and other research, with the deepening of functional genomics research, the biological function research of biological size molecules plays a very important role; the interaction analysis of biological size molecules becomes the current molecule. An important means in the study of functional science, so a good molecular interaction research tool will undoubtedly greatly promote our research.
At present, there are numerous detection technologies for studying molecular interactions. From the traditional end-point ELISA, Co-IP, and Western Blot hybridization detection, the rapid and hot real-time label-free molecular interaction systems such as Biacore, Nicoya, Fortebio, MST, and ITC have been used in recent years. The parameters obtained by the mark-free technology, including ka, kd, KD value or thermodynamics, are different, and there are many different opinions. It is dazzling. Which system is most suitable for you is a headache for everyone.
Biacore molecular interaction analyzer on tall
The Biacore Molecular Interoperator is a real-time unlabeled molecular interaction detection technology based on SPR technology developed by the Swedish company in 1990. It was acquired by the US GE in 2007 with a Swedish Krona SEK2.9 billion (US$390 million) and has since been promoted. The system uses a continuous gold film as a detection medium to detect intermolecular reactions by a change in the refractive index-based SPR angle, and parameters obtained include ka, kd, KD, protein concentration, and the like. Biacore's SPR technology has experienced nearly 20 years of development and has become the gold standard for label-free molecular interaction detection technology, and its model is rich, including Biacore8K/8K+, BiacoreS200, BiacoreT200, BiacoreX100, BiacoreC, the minimum detection limit of molecular weight from 100Da to Unrestricted, can be widely used in pharmacology, antibody vaccine screening, protein research, etc., but because the optical path system of the instrument is relatively complex, the maintenance cost is relatively high, the instrument operation is complicated and the price is high, and it needs special maintenance. At present, most of them are Platform or central laboratory procurement.
See more information about this instrument: https://
OpenSPR molecular interaction analyzer
OpenSPR is from Nicoya, Canada. It is based on the technical research background of the University of Waterloo in Canada and supported by the Canadian government. It was listed in 2015 and entered China in September 2016. It won the 2016 LSIA Award and is called the “zero technology threshold†by the industry. The next generation of SPR technology systems. Although the product's popularity in China is not as high as that of GE's Biacore, the global sales volume is very good. There are currently more than 400 units. The popularity in China is rising. Several dozens have been installed and many high-impact factors SCI have been published. The article is a very promising brand.
The company's OpenSPR uses innovative nano-gold particle-based local surface plasmon resonance (LSPR) technology, which distinguishes between traditional gold-based, refractive index-based SPR angle changes, and LSPR detects intermolecular interactions through wavelength changes. It is the next-generation SPR technology that breaks through the “zero technology thresholdâ€; the biggest feature is simple operation, fast output, easy maintenance of the instrument and detection without temperature and buffer refractive index. It can detect molecules larger than 90Da. Real-time Co-IP detection can be completed quickly in 2~3 hours; the detection parameters include Ka, kd, KD, EC50, protein concentration, etc. Compared with the past millions of molecular interoperators, the price is relatively low. Very popular with the laboratory team teacher. In 2015, only a single-channel personal version was released. In 2018, a new generation of OpenSPR single- and dual-channel/OpenSPR-XT dual-channel high-throughput upgrades was released for pharmacology, proteomics, oncology, cell signaling, and immunology. Research is a very good tool. The single and dual channel configuration is suitable for the lab to purchase and can be used as a supplement to large instruments. The new high-throughput OpenSPR-XT is suitable for instrument platforms.
See more information about the instrument: http://
High-throughput screening biofilm interference technology (BLI)
USA ForteBio molecular interaction analyzer was developed in 2006 Dr. Tan Hong listing of a technical system, which was founded in 2001 Fortebio began the development of the company, in the middle after the company almost does not survive, to save Agilent in 2004, and In 2006, the product was developed and launched, and was acquired by PALL in 2012. In 2015, PALL was acquired by Danaher. Currently, the system is divided into Meigu Molecular Instruments; its detection principle based on biofilm interference technology (BLI) The interaction between the molecules is detected by the interference of the wavelength phase shift of the membrane surface. The detection parameters include ka, kd, KD. The sensor is produced at the Shanghai factory. The current models include: Octect series RED96e, HTX, RED384, RED96, QKe, K2 and BLITZE, the actual molecular weight detection size ranges from 2KD to 100KD. Fortebio's strength lies in its high throughput, which allows rapid screening of 96 or 384 samples at the same time, but has limited molecular weight detection and is suitable for antibody vaccine screening. It is not suitable for small molecule drugs or peptide drug screening; it is compatible with SPR technology systems. Biacore or OpenSPR works together better.
More details on this product can be found at this link: https://
Isothermal titration calorimeter without sample fixation
The main brand of isothermal titration calorimeter in the market is Malvern, which uses the principle of isothermal titration calorimetry (ITC). It is an important research thermodynamics and biodynamics developed in recent years. The method continuously and accurately monitors and records the calorimetric curve of a changing process through a highly sensitive and highly automated microcalorimeter, and simultaneously provides thermodynamic and kinetic information in situ, online and without damage, and obtains kinetic parameters. KD, stoichiometry (n), 焓 (ΔH) and entropy (ΔS); instrument operation is complicated, the amount of protein is more than 10ug, the sample requires high purity, some precious samples may not meet the requirements; it does not need to be fixed Body, which has advantages in the detection of some structurally sensitive proteins; the kinetic parameters are only KD values, so it is impossible to obtain several critical parameters such as ka, kd values ​​for pharmacology, which are limited in small molecule drugs or antibody drug research applications. Instrument non-mobile phase detection, mixed sample detection is limited; another can obtain stoichiometric (n), 焓 (ΔH) and entropy (ΔS) values, these parameters for chemistry Chemical and structural analysis to be useful, so in general, isothermal titration is more suitable for laboratory research in the field of chemical synthesis and chemical structure.
Check the instrument for more information:
Https://
Micro-thermometer without sample fixation
The micro-thermometer is a product launched by NanoTemper in 2010. It uses micro-thermal mobility technology (MST), the directional movement of molecules in a microscopic temperature gradient field, and the biomolecular structure/conformation changes cause its hydration layer, Changes in molecular weight and charge, resulting in changes in the distribution of molecules in the temperature gradient field, analysis of intermolecular interactions and various chemometric parameters, including KD, energetics, by lysine autofluorescence or self-labeled fluorescence distribution changes ΔG (free energy), ΔH (焓) and ΔS (entropy), current models include NT.115 and NT.115 pico requiring fluorescent labeling and non-fluorescent labeling based on lysine autofluorescence detection of NT.LableFree and NT. Automated; system features no ligand immobilization, is superior to some structurally sensitive protein detection, and can directly detect the interaction of target proteins labeled with fluorescence in solution, but many concentration gradients are needed to fit the KD value. The overall sample requires more, although there are 16-channel and 96-channel models, but in fact only one set of KD values ​​or 8 sets of KD values ​​can be measured at a time, for high-throughput screening There are limitations in the experiment; fluorescent labeling increases the complexity and cost of the experiment, and the detection has no high and fast ELISA flux. The unlabeled detection method requires non-mobile phase detection, and requires high purity, non-luminescent compounds or small molecule drug detection. Limit, the price of the instrument is relatively expensive; overall analysis, this instrument is more suitable for the platform of membrane protein or protein structure analysis.
For more information on this product please refer to the following link: http://
Xiaobian summary:
In summary, the system involves SPR, LSPR, ITC, MST and BLI technologies; SPR technology is undoubtedly the most classic molecular interaction technology. The mainstream brands in the market are mainly GE Biacore and Nicoya. Biacore uses classic SPR technology. The instrument on the taller one is more suitable for the instrument platform; Nicoya uses the next-generation SPR technology, which is easy to operate, fast in results, flexible in configuration, suitable for both laboratory and instrument platform models; ForteBio biofilm interference technology High flux but limited molecular weight detection, has a large advantage in antibody screening, suitable for antibody research and development enterprises or platforms; MST and ITC have advantages in membrane protein and protein structure research because they do not use immobilized proteins, but also suitable for some chemistry The platform for research.
For more details, stay tuned for the next breakdown!
Xiaobian: Molecular interaction
At present, there are numerous detection technologies for studying molecular interactions. From the traditional end-point ELISA, Co-IP, and Western Blot hybridization detection, the rapid and hot real-time label-free molecular interaction systems such as Biacore, Nicoya, Fortebio, MST, and ITC have been used in recent years. The parameters obtained by the mark-free technology, including ka, kd, KD value or thermodynamics, are different, and there are many different opinions. It is dazzling. Which system is most suitable for you is a headache for everyone.
In the above figure, the interaction of the four groups of molecules is the same from the KD value, but after the subdivision, their ka and kd values ​​are very different, and the differences are hidden in the data that could not be obtained or ignored before; Only through the difference of these data can we really resolve the differences in the mechanism of action between molecules, so as to explain the mechanism of life and screen for high-efficiency drugs, which suggests that we need to pay attention to the core points of instrument purchase.
In addition to the test parameters, teachers may also want to purchase a molecular interaction system that is simple to operate, has high detection efficiency, and accurate results, but how to choose such a brand? Today, Xiaobian will give you the molecular interoperability of several mainstream brands on the market. I hope that you can help you before you break through the iron shoes. You can get all the effort today!Biacore molecular interaction analyzer on tall
The Biacore Molecular Interoperator is a real-time unlabeled molecular interaction detection technology based on SPR technology developed by the Swedish company in 1990. It was acquired by the US GE in 2007 with a Swedish Krona SEK2.9 billion (US$390 million) and has since been promoted. The system uses a continuous gold film as a detection medium to detect intermolecular reactions by a change in the refractive index-based SPR angle, and parameters obtained include ka, kd, KD, protein concentration, and the like. Biacore's SPR technology has experienced nearly 20 years of development and has become the gold standard for label-free molecular interaction detection technology, and its model is rich, including Biacore8K/8K+, BiacoreS200, BiacoreT200, BiacoreX100, BiacoreC, the minimum detection limit of molecular weight from 100Da to Unrestricted, can be widely used in pharmacology, antibody vaccine screening, protein research, etc., but because the optical path system of the instrument is relatively complex, the maintenance cost is relatively high, the instrument operation is complicated and the price is high, and it needs special maintenance. At present, most of them are Platform or central laboratory procurement.
See more information about this instrument: https://
OpenSPR molecular interaction analyzer
OpenSPR is from Nicoya, Canada. It is based on the technical research background of the University of Waterloo in Canada and supported by the Canadian government. It was listed in 2015 and entered China in September 2016. It won the 2016 LSIA Award and is called the “zero technology threshold†by the industry. The next generation of SPR technology systems. Although the product's popularity in China is not as high as that of GE's Biacore, the global sales volume is very good. There are currently more than 400 units. The popularity in China is rising. Several dozens have been installed and many high-impact factors SCI have been published. The article is a very promising brand.
The company's OpenSPR uses innovative nano-gold particle-based local surface plasmon resonance (LSPR) technology, which distinguishes between traditional gold-based, refractive index-based SPR angle changes, and LSPR detects intermolecular interactions through wavelength changes. It is the next-generation SPR technology that breaks through the “zero technology thresholdâ€; the biggest feature is simple operation, fast output, easy maintenance of the instrument and detection without temperature and buffer refractive index. It can detect molecules larger than 90Da. Real-time Co-IP detection can be completed quickly in 2~3 hours; the detection parameters include Ka, kd, KD, EC50, protein concentration, etc. Compared with the past millions of molecular interoperators, the price is relatively low. Very popular with the laboratory team teacher. In 2015, only a single-channel personal version was released. In 2018, a new generation of OpenSPR single- and dual-channel/OpenSPR-XT dual-channel high-throughput upgrades was released for pharmacology, proteomics, oncology, cell signaling, and immunology. Research is a very good tool. The single and dual channel configuration is suitable for the lab to purchase and can be used as a supplement to large instruments. The new high-throughput OpenSPR-XT is suitable for instrument platforms.
See more information about the instrument: http://
High-throughput screening biofilm interference technology (BLI)
USA ForteBio molecular interaction analyzer was developed in 2006 Dr. Tan Hong listing of a technical system, which was founded in 2001 Fortebio began the development of the company, in the middle after the company almost does not survive, to save Agilent in 2004, and In 2006, the product was developed and launched, and was acquired by PALL in 2012. In 2015, PALL was acquired by Danaher. Currently, the system is divided into Meigu Molecular Instruments; its detection principle based on biofilm interference technology (BLI) The interaction between the molecules is detected by the interference of the wavelength phase shift of the membrane surface. The detection parameters include ka, kd, KD. The sensor is produced at the Shanghai factory. The current models include: Octect series RED96e, HTX, RED384, RED96, QKe, K2 and BLITZE, the actual molecular weight detection size ranges from 2KD to 100KD. Fortebio's strength lies in its high throughput, which allows rapid screening of 96 or 384 samples at the same time, but has limited molecular weight detection and is suitable for antibody vaccine screening. It is not suitable for small molecule drugs or peptide drug screening; it is compatible with SPR technology systems. Biacore or OpenSPR works together better.
More details on this product can be found at this link: https://
Isothermal titration calorimeter without sample fixation
The main brand of isothermal titration calorimeter in the market is Malvern, which uses the principle of isothermal titration calorimetry (ITC). It is an important research thermodynamics and biodynamics developed in recent years. The method continuously and accurately monitors and records the calorimetric curve of a changing process through a highly sensitive and highly automated microcalorimeter, and simultaneously provides thermodynamic and kinetic information in situ, online and without damage, and obtains kinetic parameters. KD, stoichiometry (n), 焓 (ΔH) and entropy (ΔS); instrument operation is complicated, the amount of protein is more than 10ug, the sample requires high purity, some precious samples may not meet the requirements; it does not need to be fixed Body, which has advantages in the detection of some structurally sensitive proteins; the kinetic parameters are only KD values, so it is impossible to obtain several critical parameters such as ka, kd values ​​for pharmacology, which are limited in small molecule drugs or antibody drug research applications. Instrument non-mobile phase detection, mixed sample detection is limited; another can obtain stoichiometric (n), 焓 (ΔH) and entropy (ΔS) values, these parameters for chemistry Chemical and structural analysis to be useful, so in general, isothermal titration is more suitable for laboratory research in the field of chemical synthesis and chemical structure.
Check the instrument for more information:
Https://
Micro-thermometer without sample fixation
The micro-thermometer is a product launched by NanoTemper in 2010. It uses micro-thermal mobility technology (MST), the directional movement of molecules in a microscopic temperature gradient field, and the biomolecular structure/conformation changes cause its hydration layer, Changes in molecular weight and charge, resulting in changes in the distribution of molecules in the temperature gradient field, analysis of intermolecular interactions and various chemometric parameters, including KD, energetics, by lysine autofluorescence or self-labeled fluorescence distribution changes ΔG (free energy), ΔH (焓) and ΔS (entropy), current models include NT.115 and NT.115 pico requiring fluorescent labeling and non-fluorescent labeling based on lysine autofluorescence detection of NT.LableFree and NT. Automated; system features no ligand immobilization, is superior to some structurally sensitive protein detection, and can directly detect the interaction of target proteins labeled with fluorescence in solution, but many concentration gradients are needed to fit the KD value. The overall sample requires more, although there are 16-channel and 96-channel models, but in fact only one set of KD values ​​or 8 sets of KD values ​​can be measured at a time, for high-throughput screening There are limitations in the experiment; fluorescent labeling increases the complexity and cost of the experiment, and the detection has no high and fast ELISA flux. The unlabeled detection method requires non-mobile phase detection, and requires high purity, non-luminescent compounds or small molecule drug detection. Limit, the price of the instrument is relatively expensive; overall analysis, this instrument is more suitable for the platform of membrane protein or protein structure analysis.
For more information on this product please refer to the following link: http://
Xiaobian summary:
In summary, the system involves SPR, LSPR, ITC, MST and BLI technologies; SPR technology is undoubtedly the most classic molecular interaction technology. The mainstream brands in the market are mainly GE Biacore and Nicoya. Biacore uses classic SPR technology. The instrument on the taller one is more suitable for the instrument platform; Nicoya uses the next-generation SPR technology, which is easy to operate, fast in results, flexible in configuration, suitable for both laboratory and instrument platform models; ForteBio biofilm interference technology High flux but limited molecular weight detection, has a large advantage in antibody screening, suitable for antibody research and development enterprises or platforms; MST and ITC have advantages in membrane protein and protein structure research because they do not use immobilized proteins, but also suitable for some chemistry The platform for research.
For more details, stay tuned for the next breakdown!
Xiaobian: Molecular interaction
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