Pond nesting squid practical technology

In recent years, the fishermen of the Binhu area in our county have kept a close eye on the domestic and foreign markets of squid and have used the advantages of rich wild fish and shrimp resources to find a practical technique for raising squid in a pond and have produced good results. The benefits are significant. By adopting this type of cultivation, not only can control the breeding of wild fish, and avoid wild bait fish and main fish cultivating bait, fighting for oxygen, and fighting for space, thereby accelerating the growth rate of the main fish, and improving the utilization rate of feed. Reduce the spread of some fish diseases, because diseased fish are slow in movement and are easily eaten by carp, thus eliminating the source of infection and preventing the spread of the disease. Now this farming method is introduced as follows: 1. Pond requirements. Set up a pond for squid, the bottom of the pool requires that the silt thickness should be less than 20 cm, preferably a sandy bottom, and some water plants. The water source is convenient, and the water is fresh and pollution-free. Every year, dry ponds should be used to remove excess silt, and clear water ponds should be disinfected with 150 kg of lime per acre 15 days before the fish species are released. After 5 days, pool water should be added to the deepest level. Second, set the way. Squid seedlings can be placed at the same time as the main fish. Each acre is accompanied by 20 squids with a tail weight of about 50 grams. The size of the main fish is larger, and each weighs more than 100 grams. Because the growth of carp is faster than that of the main fish, it is necessary to widen the gap in the size of the fish species, and to avoid the later small food, which will endanger the main fish culture. Oysters have poor oxygen tolerance. The pond that raises the squid is mainly cultivated grass carp, and it is also properly equipped with fertilizer fish. It has 20 oysters per mu and 10 oysters to consume nutrients in the water to improve water quality. Some oysters, clams, tilapia, etc., allow them to spawn naturally and hatch fry for squid. At the same time, oysters, clams, and tilapia can feed the residual baits or rot fish at the bottom of the pool to prevent deterioration of water quality. Third, the bait feed. Feeding amount of bait fish should be determined by the amount of wild fish in the pond and the number of squid rearing. In the Binhu area of ​​our county, there are many wild fishes in the pond. Feeding bait fish, this is a very economic and reasonable way of stocking. If there is a shortage of wild fish in the pond, a small group of small wild fish can be put into the pool to allow them to breed offspring so as to ensure that the squid has sufficient feed fish every day, and the stocking ratios of quail, oyster and tilapia may be appropriately increased. . In the case of a small amount of miscellaneous fish, put a few kinds of carp species, which are widely sourced and low in price, into bait fish every day; the amount of each carp is about 5 fish per day. Fourth, daily management. Carp enjoys a clean flowing water environment. It is necessary to inject new water frequently to increase the oxygen in the pool and maintain microfluidic conditions. In the hot season, pay attention to the prevention and treatment of fish diseases. In the period from June to September, 25 kilograms of lime should be applied every 15 days to regulate water quality and kill bacteria. Fish ponds should be used with caution, such as mercurous nitrate, trichlorfon, and malachite green. Kill parasites, bacteria, etc. 0.8ppm copper sulphate and ferrous sulfate mixture and 1ppm bleach or 10ppm formalin Quanchiposa

Extraction Kit Magnetics Bead Method

This standard specifies the terms and definitions, classification, technical requirements, test methods, identification, labels and instructions for use, packaging, transportation and storage of nucleic acid extraction kits (magnetic beads method) (hereinafter referred to as "kits"). This standard It is suitable for extracting and purifying human genomic nucleic acid and its fragments from various clinical samples such as serum, plasma, whole blood, cerebrospinal fluid, milk, saliva, urine, sputum, swab, tissue or paraffin-embedded tissue by magnetic bead method , Pathogen nucleic acid kit. Pathogen nucleic acid includes deoxyribonucleic acid (DNA), ribonucleic acid (RNA), etc. This standard does not apply to nucleic acid extraction reagents contained in closed systems that cannot extract nucleic acid extraction products for detection.

This kit uses magnetic beads with unique separation function and a unique buffer system to separate and purify high-quality virus from serum, plasma, lymph, cell-free body fluid, cell culture supernatant, urine or various virus preservation solutions DNA/RNA. The uniquely embedded magnetic beads have a strong affinity for nucleic acids under certain conditions, and when the conditions change, the magnetic beads release the adsorbed nucleic acids, which can achieve the purpose of rapid separation and purification of nucleic acids. The whole process is safe and convenient, and the extracted viral DNA/RNA has high yield, high purity, stable and reliable quality, and is especially suitable for automated extraction of high-throughput workstations. The nucleic acid purified by this kit can be applied to various routine operations, including RT-PCR, fluorescence quantitative PCR and other downstream experiments.

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