There are a wide variety of bovine serum albumin (BSA) products for different applications. But how to choose it, let Epson take you to solve this problem together!
At present, BSA is roughly classified into standard grade BSA, BSA fifth component, IgG-free reagent grade BSA, protease-free BSA, fatty acid-free BSA, low endotoxin BSA, protease-free/IgG BSA, and diagnostic grade BSA.
Let's take a look at their differences:
BSA type | Product features and applications |
Standard grade BSA | The heat-treated ordinary BSA product can be used as a nutrient and culture component for tissue cells (microorganisms, animal and insect cells, etc.) and a protein molecular weight standard component, and is a cost-effective BSA product. |
BSA fifth component | The fifth component of BSA is more pure than ordinary BSA. The fifth component of BSA is purified using the Cohn method and is a biotech grade product, generally used for cell culture, as a hapten carrier, a protective agent, and a protein supplement control. |
No IgG reagent grade BSA | The reagent grade BSA has better purity than the fifth component of BSA, which does not contain globulin IgG components that may interfere with the analysis. It can be used as a cell culture nutrient and culture component as well as a protein molecular weight standard component. |
Protease-free BSA | Protease-free bovine serum albumin can be used in cell culture to produce protein assays, particularly for expressing proteins that are sensitive to protease activity. In addition, protease-free bovine serum albumin is also very suitable. |
Fatty acid free BSA | Fatty acid-free BSA can be used as a carrier protein for fat metabolism-related analysis, or combined with a specific fatty acid to form albumin, which can be used to enhance the performance of cell culture systems; if the presence of fat or fatty acids in the experiment affects hormones or cholesterol This product is preferred when reading. |
Low endotoxin BSA | Low endotoxin bovine serum albumin is an ideal choice for cell and tissue culture. It can be used as a carrier for serum-free and synthetic media supplements. It is also used as a general reagent in the laboratory to stabilize and dilute sensitive protein solutions. It can also be used as a diagnostic and analytical tool such as ELISA. |
Protease-free/IgG BSA | It does not contain protease and RNase enzyme activity, and does not contain IgG. It has a higher purity grade and is used as a diluent or blocking solution in tests such as EIA and ELISA. |
Diagnostic BSA | Diagnostic grade bovine serum albumin powder is specifically designed to reduce factors such as background interference and inhibition of cell culture systems, without proteases, bioburden and IgG, with very low activity of endotoxin (less than 3.0 EU/mg). It can be used for protein standards, diluents, conjugates or enzyme stabilizers, as well as for highly sensitive immunoassay experiments, cell culture, and hybridization experiments. |
BHP's origin of BSA originated in Melbourne, Australia. Since 2001, the whole process of closed-loop processing has been used to produce bovine serum albumin. In recent years, it has expanded its production scale to provide a range of high quality animal serum and protein products. These products use Australian and New Zealand plasma, which is not only popular in the market, but also avoids diseases such as mad cow disease, chronic wasting disease, rabies, hand, foot and mouth disease and herpes stomatitis.
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