When doing immunoprecipitation experiments, in many cases, when encountering the problem of heavy chain light chain, I don't know which second antibody is better. I know that a good secondary antibody product can guarantee the accuracy of the experimental results. , then choose IPkine specific antibodies can be avoided, the following is the experience:
In order to avoid heavy chain interference in the experiment, Aimei recommends two solutions.
One is to select the primary antibody of different species to carry out IP and WB experiments, and then select the secondary antibody without cross-reaction to carry out WB experiment; this scheme is relatively expensive, need to buy two primary antibodies and two Corresponding secondary antibodies.
The other is to use the same antibody in IP and WB experiments. If the target band is around 55 kDa, the interference of the heavy chain band on the detection of the target protein can be eliminated by selecting the secondary antibody that specifically binds to the light chain.
IPkine specific antibody advantages:
Clean background, clear signal
Can effectively eliminate the target protein signal concealment caused by denatured heavy chain interference;
Good reactivity:
The IPkine-specific antibody specifically reacts with the light chain and undergoes special treatment with little cross-reactivity with the heavy chain molecule.
Widely applicable
Amytech IPkine specific antibody is available in a variety of antibodies, suitable for rabbit, mouse, rat, goat or sheep source;
Easy to use
easy to use! Simply replace the Amytech IPkine-specific antibody with a traditional secondary antibody. In addition, Amytech IPkine specific antibodies are available in stock, and can also use Amytech IPkine specific antibody products.
The American Abbkine brand is a brand that provides cost-effective immunology and cytology research products and solutions. Amy Jie introduced it to China as its Chinese IPkine series of secondary antibodies - effectively removing WB heavy chain interference after immunoprecipitation IP.
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