First of all, Xiaobian will give you a general science, the common diseases and diagnosis methods of dogs. Among all diseases of dogs, canine distemper virus, rabies virus, canine parvovirus, canine coronavirus, and canine herpes virus account for more than 50% of canine pathogen diseases. Currently on the market with the most is colloidal gold immunoassay test strip, to diagnose the disease by detecting an antigen or antibody, simple operation, the results may be observed within 5-10min. However, the test strip has certain defects, and it is prone to false negatives and false positives. In the early stage of the disease, the antibody level has not reached the detection limit, and it is prone to false negatives; after the disease is cured, the antibody still exists, and the test results are false positive. In addition, when the test result is weakly positive, it indicates that the disease may or may not be diagnosed, and the diagnosis cannot be obtained. Therefore, the method has also been questioned. By visiting the pet hospital, we found that pet doctors generally responded that the test strip detection accuracy was too low. Many cases that were judged by experience or other tests were tested with test strips, and the results were still negative, which became the number one problem for pet doctors. .
In recent years, molecular diagnostic methods for direct detection of pathogen genes have arisen. Polymerase chain reaction (PCR) has also gradually occupied the disease diagnosis market for molecular detection. The sensitivity and specificity of PCR detection results are higher than that of test strips, but the relative operation is complicated. It requires the steps of sample preparation, nucleic acid extraction, nucleic acid amplification, detection, etc., and needs to be equipped with a complicated operation instrument and detector. It requires a professional molecular operator, which increases the difficulty and cost of detection, and is not suitable for general use. Used by pet hospitals.
Is there any product on the market that is fast, accurate and simple for pet pathogen detection?
Good news is coming! ! ! ! Our company has developed a new generation of nucleic acid molecule detection technology-SEA. SEA products directly detect the pathogen gene, and the accuracy is similar to PCR, but the operation is simpler. The result can be judged by macroscopic colorimetry or fluorescence in about 20 minutes. A product between PCR and test strips.
The application of SEA technology product testing samples can be divided into three steps:
1) nucleic acid cleavage: the sample is added to the lysate and thermally lysed;
2) Nucleic acid amplification: Add buffer A, buffer B, buffer C and template to the tube and amplify it.
3) Result interpretation: The report is printed directly by color interpretation results or by machine.
At the same time, this product has the following obvious advantages, superior to the market products:
1) Multiple targets: The first DNA/RNA one-step method in the world, the test results are more accurate;
2) Isothermal detection: the detection process temperature is constant and the system is stable;
3) Lower cost: the reaction system is simple, no complicated instruments are needed, and the cost is effectively reduced by more than 50%.
If you are still worried about the inaccurate test results of the test strips, if you are still troubled by the choice of testing methods, you may wish to try our products, you will certainly receive satisfactory results. If you have no corresponding products in the market for animal diseases, you can also contact us, we can provide you with integrated nucleic acid testing services to protect your life and health!
Qingdao Naide Biotechnology Co., Ltd.
----One-stop supply of nucleic acid detection products
Contact: Ma Jiapeng
phone
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