There is less than a month to the Spring Festival, cold low temperature days, rainy days, smog days and other illuminating period, the humidity in the shed is relatively large, so in the case of low temperature and low temperature, the shed treatment should pay attention to What are you? The vegetable farmers must pay attention to the following four points.
1 temperature
During the winter time, the night temperature cannot be controlled too high. According to the meteorological conditions, the grass curtain should be placed as late as possible in the afternoon, and the grass curtain should be pulled early every morning to keep the temperature difference between day and night, and provide more light time for vegetables. .
2 humidity
In this weather situation, the humidity in the shed is usually relatively large. Therefore, it is necessary to keep the ventilation in the shed as much as possible, and to ventilate as much as possible to minimize the humidity in the shed. In addition, watering in winter should try to choose drip irrigation or micro-spraying methods to avoid flood irrigation. In the shed without drip irrigation equipment, water should be poured to keep the soil moisture in the shed should be controlled at 50% to 70%.
3 pruning
In the winter, there are more rainy, snowy days and smog days. Therefore, when carrying out cropping operations such as pruning and smashing, try to avoid this weather and choose to do it in good weather at noon and in a relatively high temperature period. To promote wound healing. If the foggy days and other illuminating days must be operated, wait until the humidity in the shed is lowered, and then spray after the operation to avoid the occurrence of diseases.
4 spray
At present, it is precisely the time period when many diseases are extremely easy to occur. It is necessary to spray drugs on time to prevent the occurrence of various diseases as early as possible. The vegetable farmers can alternately spray according to the weather conditions before the continuous rainy and snowy days, such as chlorothalonil, methyl thiophanate, chunleimycin, etc., and it is better to add carapace when spraying. Vegetarian and amino acid foliar fertilizers enhance plant resistance.
PCR ( Polymerase Chain Reaction)
It provides a way to make more copies of a portion of DNA.
We need to have PCR ingredients that include DNA portion, Buffer solution, Primer+DNA (tap polymerase), and Nucleotides during the preparation step.
In the step of PCR sequence, there are three steps in this section.
Step 1 Denaturation
The addition of heat is needed to separate the two strands of the DNA molecule.
Step 2 Annealing
Temperature, is going to be cool in this step, should allow the primers to bind to the specific segments.
Step 3 DNA Synthesis
To make more copies of the DNA.
Those three steps above keep repeating to make enough copies of the DNA fragments. It is the principle of how PCR machine works.
Superyears General 2215 RT-qPCRadopts all-in-one with an industrial art design, combining the Analytical Instrument, Large touch screen, and Computer. General 2215 RT-qPCR is equipped with the proven thermal-cooled electric technology, which can recognize the speed rate of temperature control faster and obtain high-quality testing results within 40 minutes. To ensure the sensitivity and stability of the testing a design with traditional beam path with a susceptible CMOS image sensor is used. In addition, General 2215 supports multiple PCR analysis and High-resolution Melt Curve (HRM) analysis, compatible with the mainstream reagent kit in the market. It will be the best assistant and your preferred RT-qPCR.
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