Model NO.: CRE
Application: Hospital, Lab, School
Trademark: Biomedia
Specification: R1: 2*60 mL, R2: 2*20 mL
Origin: Jinan, Shandong
Model NO.: CRE
Application: Hospital, Lab, School
Trademark: Biomedia
Specification: R1: 2*60 mL, R2: 2*20 mL
Origin: Jinan, Shandong
The creatinine (CRE) assay kit is intended for the accurate and direct quantitative measurement of CRE in biological fluids such as serum. Blood CRE is used to determine glomerular filtration rate (GFR), a measure of kidney function.Testing Principle
During the assay, CRE is converted to creatine by creatininase. Creatine is converted to sarcosine in the presence of creatinase. Sarcosine is then oxidized, and the product reacts with the probe, generating red quinochrome, concentration of which can be determined by the colorimetric method. The concentration of CRE can be calculated from that of red quinochrome.
Product Features
- Linearity range: 2 - 880 μmol/L; linear correlation coefficient ≥0.990; linearity deviation is within ±10%.
- Absorbance of blank ≤ 0.50
- Sensitivity: the difference of absorbance per unit of concentration (ΔA) ≥ 0.10 while testing the specific sample of concentration of 442 μmol/L.
- Accuracy: Relative deviation is within ±10%.
- Precision: CV ≤ 5%; precision between batches ≤ 5%.
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Main Wavelength | Secondary Wavelength | Method | Reaction Direction | Sample Volume | R1 Volume | R2 Volume | Reaction Time |
546 nm | 700 nm (optional) | End point method | Bottom-up | 4 μl | 240 μl | 80 μl | 300 s |
Packaging Specification
R1: 2×60 ml, R2: 2×20 ml
R1: 4×60 ml, R2: 4×20 ml
R1: 1×30 ml, R2: 1×10 ml (Sample)
Calibrator: appendant
Quality control: additional purchase
Storage and Period of Validity
Unopened: Store assay kits at 2 - 8ºC protected from light. Stable to expiration date printed on individual vials (for 16 months).
Opened: Store assay kits at 2 - 8ºC protected from light. Stable for 1 month if kept capped from original container and free from contamination.
Do not freeze the assay kit.
 The creatinine (CRE) assay kit is intended for the accurate and direct quantitative measurement of CRE in biological fluids such as serum. Blood CRE is used to determine glomerular filtration rate (GFR), a measure of kidney function.
Testing Principle
During the assay, CRE is converted to creatine by creatininase. Creatine is converted to sarcosine in the presence of creatinase. Sarcosine is then oxidized, and the product reacts with the probe, generating red quinochrome, concentration of which can be determined by the colorimetric method. The concentration of CRE can be calculated from that of red quinochrome.
Product Features
- Linearity range: 2 - 880 μmol/L; linear correlation coefficient ≥0.990; linearity deviation is within ±10%.
- Absorbance of blank ≤ 0.50
- Sensitivity: the difference of absorbance per unit of concentration (ΔA) ≥ 0.10 while testing the specific sample of concentration of 442 μmol/L.
- Accuracy: Relative deviation is within ±10%.
- Precision: CV ≤ 5%; precision between batches ≤ 5%.
Â
Main Wavelength | Secondary Wavelength | Method | Reaction Direction | Sample Volume | R1 Volume | R2 Volume | Reaction Time |
546 nm | 700 nm (optional) | End point method | Bottom-up | 4 μl | 240 μl | 80 μl | 300 s |
Packaging Specification
R1: 2×60 ml, R2: 2×20 ml
R1: 4×60 ml, R2: 4×20 ml
R1: 1×30 ml, R2: 1×10 ml (Sample)
Calibrator: appendant
Quality control: additional purchase
Storage and Period of Validity
Unopened: Store assay kits at 2 - 8ºC protected from light. Stable to expiration date printed on individual vials (for 16 months).
Opened: Store assay kits at 2 - 8ºC protected from light. Stable for 1 month if kept capped from original container and free from contamination.
Do not freeze the assay kit.
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