Abstract: Ten carbamate pesticides and their metabolites were determined by NY/T 761-2008 standard method (post-column derivatization high performance liquid chromatography).
Key words: carbamate, post-column derivatization, vegetables and fruits, pesticide detection
Carbamates are widely used as insecticides, herbicides, fungicides, and the like. It inhibits the accumulation of acetylcholine (Ach) and carboxylate by inhibiting the activity of insect acetylcholine enzyme (Ache) and carboxylesterase, which affects the normal nerve conduction of insects and causes death.
The third part of the NY/T 761-2008 standard issued by the Ministry of Agriculture on April 30, 2008 specifies the multi-residue liquid chromatography method for the determination of 10 carbamate pesticides and their metabolites in vegetables and fruits: amino The formate pesticide is hydrolyzed on-line after column separation to form methylamine, which is then derivatized by fluorescence and detected by a fluorescence detector. According to this method, 10 kinds of carbamate pesticides and their metabolite mixed standards were measured.
instrument:
LabAlliance binary high pressure gradient system with fluorescence detector, equipped with Lanbo Rambo third generation post-column derivation system , OV2001 column oven , column: SMT-Elite-C18 (4.6 mm × 25 mm, 5 μm), A post-column derivatization reagent package.
Analysis conditions:
Column temperature: 42 ° C
Fluorescence detector wavelength: λex=330 nm, λem=465 nm
Mobile phase: A: water B: methanol
Gradient (A %/T): 85/0, 75/2, 75/8, 60/9, 55/10, 20/19, 20/25, 85/26
Derivatization reagent: hydrolysis: 0.05 mol/L NaOH solution, 0.3 mL/min, 100 °C
Derivatization: OPA solution prepared with 0.4% sodium borate solution containing OPA 100 mg/950 mL, mercaptoethanol 2 g/950 mL, 0.3 mL/min, room temperature.
Injection volume: 10 μL
Standard chromatogram:
1 - azide sulfoxide
2—Adicarb sulfone
3—Dedomone
4-trihydroxycarbone
5 - Aldicarb
6-speed
7—Capeway
8-naphthyl
9-isoprocarb
10—Zhong Dingwei
For more information, please visit the website of Tianjin Rambo:
Or Rambo Chromatography Mall:
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