Revealing the Mechanism of Gene Editing Tools SpCas9
February 15, 2019 Source: Science and Technology Daily Author: Lili Yun Yi Xiaofeng Zhu Yuwei
Window._bd_share_config={ "common":{ "bdSnsKey":{ },"bdText":"","bdMini":"2","bdMiniList":false,"bdPic":"","bdStyle":" 0","bdSize":"16"},"share":{ }};with(document)0[(getElementsByTagName('head')[0]||body).appendChild(createElement('script')) .src='http://bdimg.share.baidu.com/static/api/js/share.js?v=89860593.js?cdnversion='+~(-new Date()/36e5)];The reporter learned from Harbin Institute of Technology that a project completed by Huang Zhiwei, a professor at the School of Life Sciences, "The Structural Basis of the High-Fidelity SpCas9 Variant", was published in the latest international journal Cell Research. This achievement not only reveals the molecular mechanism of the high-fidelity Cas9 gene editing system for the first time, but also transforms SpCas9 and other Cas endonuclease enzymes into a more efficient and specific gene editing tool to consolidate the key structural basis and guide the transformation. The application value of the new gene editing system is expected to treat hereditary diseases and difficult diseases.
The CRISPR-Cas9 system is used as the most widely used gene editing tool in the fields of biology and medicine, and can edit genes. At present, through the efforts of scientists in the field of global gene editing, high-fidelity SpCas9 mutants have been produced on the basis of Cas9, and these mutants can become more efficient and convenient gene editing tools and systems. But the off-target effect is the biggest damage of this efficient gene editing tool, and it will bring unexpected mutations, making it difficult for humans to precisely control when and where the gene editing tools are edited.
On the one hand, the above studies illustrate the molecular mechanism of the high compatibility and high fidelity of the substrate recognition substrate of SpCas9 by structural biology. On the other hand, more mutants are rationally designed to further enhance the compatibility and protection of the substrate. True.
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