Effects of Ganoderma Lucidum on Learning and Memory and Monoamine Neurotransmitters in Mice

Abstract : Objective : To observe the effect of Ganoderma lucidum on the intelligence of mice. Method: Ganoderma decoction 100g body weight per 1m l (low dose group 5gökg, high dose group 10gökg) intragastrically 2 weeks assay of learning and memory and Monoamine neurotransmitters. RESULTS : Compared with the control group , Ganoderma lucidum significantly increased the content of 52 serotonin and dopamine in the brain of mice (P < 0.05), significantly enhanced the learning ability of mice (P < 0.01), and spontaneous activity in mice. Has a certain inhibition. Conclusion : Ganoderma lucidum has obvious anti-aging and anti-aging effects.

Key words : Ganoderma lucidum ; learning ; memory ; neurotransmitter

Ganood erm alucid um K a rst. is a fungus of the family Polyporaceae. According to the "Compendium of Materia Medica" , it can fill the five dirty air , nourish and strengthen , and strengthen the foundation. Many literature reports that Ganoderma lucidum has an immune-enhancing effect , which improves the ability of learning and memory in mice and delays aging . However, studies on the effects of Ganoderma lucidum on neurotransmitters in the brain have rarely been reported. This observation Ganoderma decoction of spontaneous activity, learning and memory and monoamine neurotransmitters, to investigate the pharmacological effects of Ganoderma on improved intelligence.

1 Materials and methods

1.1 Ganoderma lucidum artificially cultivated for testing , provided by Jinhua Pharmaceutical Company. Decoction prepared according to the literature.

1.2 Experimental animals 30 Kunming mice, male and female, body weight (30 ± 4) g, provided by Experimental Animal Center Jinhua. First divided male and female mice, and then were randomly divided into 3 groups of 10 each, free access to food (diet) and water. The dose of Ganoderma lucidum in experimental group A was 5gö(kg · d), and the dose of Ganoderma lucidum in experimental group B was 10gö(kg · d) . The control group was filled with distilled water. 20g mouse body weight orally in an amount of 0. 2m l. After 16 days of irrigation , the cervical vertebrae were dislocated and killed , and the brain was quickly weighed on an electronic balance.

1. 3 Opening behavior After observing Ganoderma lucidum for 13 days , the opening behavior was observed. The bottom surface of the opening environment is divided into 13 grids , and the number of grids that the mouse runs within 3m in , the number of hind legs standing ( including the buttress standing and free standing ) , the number of grooming, and the number of stools are recorded.

1.4 spatial discrimination learning and memory after administration Ganoderma 14d, for measuring learning and memory "Y" type electric Method lost. Specific method : The “ Y ” type electric labyrinth is in a safe signal state . After the mouse touches a few times, it is put into the electric labyrinth , let it adapt to 3m in, and starts to use the random table to perform electric shock stimulation . After 10 consecutive times, rest for 1m in, to training 10 times 9 times of learning criterion of correct responses, was recorded for each mouse to achieve the desired number of training Institute standards. 24h post-test memory, 10 times in training on behalf of the percentage of correct memory retention.

1.5 brain serotonin and dopamine Determination of serotonin and dopamine by chemiluminescence.

1. 6 Statistical analysis using t 'test.

2 results

2. The effect of Ganoderma lucidum on the opening behavior of mice After the Ganoderma lucidum decoction for 13 days , the appearance and activity of the animals did not change much. Table 1 shows that compared with the control group , the number of running, number of standing, number of grooming and number of feces in the Ganoderma lucidum group decreased in 3 m in , but the difference was not significant. It is indicated that Ganoderma lucidum has a certain inhibitory effect on the spontaneous activity and inquiry behavior of mice in new and different environments.

2. Effects of 2 Ganoderma lucidum on learning and memory in mice Table 2 shows that compared with the control group , the number of learnings in the Ganoderma lucidum group was significantly reduced , but had no effect on memory. It indicated that Ganoderma lucidum significantly promoted the learning ability of mice.

2.3 Effect of Ganoderma results serotonin and dopamine content in mouse brain (Table 3) indicate that the low dose group ganoderma brain DA, 5- H T content of the control group did not differ significantly, but there are numerically increased to some extent; and mouse brain DA high doses of Ganoderma lucidum, 5- H T content significantly higher than the control group. Tip Ganoderma can significantly increase the amount of serotonin in the brain and dopamine. 3 Discussion The advanced functions of brain, such as thinking, learning and memory, are closely related to the content of central neurotransmitters. During the decline of brain function , the content of various neurotransmitters is reduced. Drugs can be made by affecting the content of certain neurotransmitters

The role of the puzzle. Monoamine neurotransmitters of attention, memory and ability to respond has an important regulatory role, epinephrine, norepinephrine and dopamine reduction in content is an important cause of learning and memory deficits. In this test , high doses of Ganoderma lucidum decoction can significantly increase the content of DA and 5-H T , which is significantly improved with high dose of Ganoderma lucidum.

The learning and memory abilities of mice are consistent ; low doses of Ganoderma lucidum increase the levels of DA and 5-H T in the brain , which is not significant , and the low dose of Ganoderma lucidum only significantly improves the learning ability of mice but does not improve the memory ability. Significantly corresponding. This indicates that Ganoderma lucidum can promote the learning and memory ability of mice by affecting the level of neurotransmitters in the brain, and inhibit the spontaneous activity of mice.

move. The present results show that the content of Ganoderma lucidum can significantly improve the mouse brain dopamine and serotonin neurotransmitters, can significantly enhance the ability of mice to learn, Ganoderma described having anti nootropic effect.