Human calcitonin ELISA kit
 ( for serum, plasma, cell culture supernatants and biological fluids )
principle
This experiment used double antibody sandwich ABC-ELISA. Coated with anti-human Calcitonin monoclonal antibody on the plate, standard and sample Calcitonin combined with monoclonal antibody, biotinylated anti-human Calcitonin, forming immune complexes attached to the plate, horseradish peroxidase The labeled Streptavidin is combined with biotin, the substrate working solution is blue, and finally the stop solution sulfuric acid is added. The OD value is measured at 450 nm, and the Calcitonin concentration is directly proportional to the OD value. The concentration of Calcitonin in the sample can be obtained by drawing a standard curve.
Kit composition ( 2-8 ° C preservation)
Coated Wells | 96 holes | Enzyme Conjugate | 12ml |
10× specimen dilution (Sample Buffer) | 12ml | 20×Wash Buffer | 50ml |
Standards: 200ng/bottle | 2 bottles | Substrate working fluid (TMB Solution) | 12ml |
Primary antibody working solution (Biotinylated Antibody) | 12ml | Stop Solution | 12ml |
Prepare reagents and collect blood samples
1. Collection of specimens: serum, plasma (EDTA, citrate, heparin anticoagulation), cell culture supernatant, tissue homogenate, etc., as early as possible, stored at 2-8 ° C for 48 hours; longer time must be frozen (-20 ° C Or -70 °C) to avoid repeated freezing and thawing.
2. Standard solution preparation: Add 1 ml of distilled water before use and mix well to prepare a 200 ng/ml solution. Set the standard tube 8 tube, the first tube plus the standard dilution 900ul, the second to the eighth tube to add the sample dilution 500ul. Add 100 ul of 200 ng/ml standard solution to the first tube, mix and aspirate 500 ul with a pipette, and transfer to the second tube. Repeat the dilution as described above, and remove 500 ul from the seventh tube and discard it. The eighth tube is a blank control.
3. The 10× specimen dilution was diluted 1:10 with distilled water (example: 1 ml concentrated dilution + 9 ml distilled water).
4. Washing solution: diluted 1:20 with distilled water (example: 1 ml concentrated washing solution added to 19 ml of distilled water)
Test procedure
1. Loading: Add 100 ul of standard or sample to be tested in each well. Mix the reaction plate thoroughly and let it stand at 37 °C for 120 minutes.
2. Wash the plate: Wash the plate thoroughly with washing solution 4-6 times, and dry it on the filter paper.
3. Add 100 ul of the first antibody working solution to each well. The reaction plate was thoroughly mixed and placed at 37 ° C for 60 minutes.
4. Wash the board: the same as before.
5. Add 100 ul of enzyme-labeled antibody working solution per well. The reaction plate was placed at 37 ° C for 30 minutes.
6. Wash the board: same as before.
7. Add 100 ul of substrate working solution to each well and let it react at 37 ° C for 15 minutes in the dark.
8. Add 100 ul of stop solution to each well and mix.
9. Measure the absorbance at 450 nm using a microplate reader within 30 minutes.
Result calculation and judgment
1. All OD values ​​should be subtracted from the blank value before calculation.
2. Using standard products 20, 10, 5, 2.5, 1.25, 0.625, 0.312, 0 ng/ml as the abscissa and OD as the ordinate, plot on the coordinate paper and draw the standard curve.
3. Find the corresponding Calcitonin content on the graph based on the OD value of the sample.
Kit performance
1. Sensitivity: The minimum concentration of Calcitonin is less than 0.15 ng/ml.
2. Specificity: Recombinant or natural human Calcitonin can be detected simultaneously. Does not cross-react with other human cytokines.
3. Repeatability: The coefficient of variation in the plate and plate is less than 10.2%.
Precautions
1. It is recommended to make double holes for the above standard holes and samples to be tested. The standard curve should be made at the same time for each measurement.
2. The washing process is critical. Insufficient washing will result in an accuracy error and an erroneous rise in the OD value.
3. After the slats are opened, the remaining slats should be sealed again to keep the slats dry .
4. This kit should be stored in a 4oC refrigerator.
5. This kit is for scientific research only and cannot be used for clinical diagnosis!
AKD Special Starch
Application: used in AKD Emulsifier
Product Type: Cassava starch imported from China
Chemical composition: Tapioca starch
Appearance:White powder
Moisture ( % ): ≤ 14.0
Spots ( a / cm2): ≤ 2
Viscosity: 20 ~ 50 s (20%, 40 °C, 4 # Tu's cup)
pH value: 6.0 ~ 7.5
Toxicity: No harm to human health during operation and use
Storage: Store in a cool , dry, ventilated warehouse
Nature: white, gelatinization in low temperature , good fluidity , viscosity stability, aging resistance , the film is good , strong bond , fast drying . Products with low protein content , gelatinization liquid not perishable , no foam , no smell. Good adhesion properties of various fibers , high compatibility with other additives . For AKD emulsifier, the rational use of the product can effectively ensure emulsifying effect and reduce production cost.
Dosage: Production of one ton of 25% emulsifier simply add 192kg of the product
Packing: 25 kg / bag.
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Product
Introduction :
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Specification of AKD Special Starch
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Application
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Applied to the AKD emulsion
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Production type
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Modified imported cassava starch from
Thainland
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Chemical component
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Cassava starch
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Appearance
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White powder
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(%)Moisture
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≤14.0
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Speckle per cm2
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≤2
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PH value
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6.0~7.5
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Fineness
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≥98 %(100Mesh
fineness)
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Whiteness
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≥90
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Viscosity
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20~50 s( 20 %,40 ℃,4#Tushi Cup)
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Toxicity
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Harmless to human
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Storage
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In cool, dry and ventilated warehouse
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Properties
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High whiteness, low pasting temperature, good
futility, ageing resistance and film-processing ability, strong bond
stress, quick to dry;low
protein content, the paste is difficult to decay, foam free and free from
extraneous odor;good adhesiveness to fiber and
good solubility with other aiding agent. Used in AKD emulsion properly and guarantee
emulsion effection and cut cost.
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Dosage
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192kg for 1ton of 25% emulsion
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Packing and transportation
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25kg /bag
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Akd Special Starch,Oxidized Starch,Starch For Akd Emulsifer,Cassava Starch
Shandong Tiancheng Chemical Co., Ltd. , https://www.tianchengchemical.com